• Seahorse Xf Cell Mito Stress Test Kit User Guide
• Xf Glycolysis Stress Test Kit User Manual Pdf

Agilent Seahorse XF Glycolysis Stress Test Kit User Guide 7 Figure 1 Agilent Seahorse XF Glycolysis Stress Test profile of the key parameters of glycolytic function. Sequential compound injections measure glycolysis, glycolytic capacity, and allow calculation of glycolytic reserve and nonglycolytic acidification. Apr 26, 2017  Immunosuppressive capacity of mesenchymal stem cells correlates with metabolic activity and can be enhanced by valproic acid. Final concentration 100 mM; Seahorse Bioscience) were performed as described in the XF Glycolysis Stress Test Kit User Manual (Seahorse Bioscience). All media and solutions were prepared and applied as recommended.

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Seahorse Xf Cell Mito Stress Test Kit User Guide

• The assay measures basal glycolysis and glycolysis capacity upon blockage of mitochondrial ATP production using respiratory modulators.
• Non-glycolytic acidification is assessed through glucose starvation
• Compounds are reconstituted directly in assay medium – eliminating the need for DMSO or other solvents.
• Kits are rigorously tested and validated for every lot with cells to deliver consistent and robust results – eliminating the cost associated with failed experiments.
• Data analysis tool, the Seahorse XF Glycolysis Stress Test Report Generator, is provided to simplify data processing by automatically calculating the key parameters of your samples.
• Download Seahorse XF Glycolysis Stress Test Report Generator Report Generator
• For use with an XFp Analyzer

The Seahorse XF Glycolysis Stress Test Kit measures the capacity of the glycolytic pathway by driving cells toward glycolysis and assesses the ability of study models to increase glycolytic activity to meet metabolic and bioenergetic demands. Refer to the Seahorse XF Glycolytic Rate Assay for a measure of lactate-associated proton efflux.
Learn more about measuring glycolysis with Seahorse XF technology

For Research Use Only. Not for use in diagnostic procedures.

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Xf Glycolysis Stress Test Kit User Manual Pdf

• The assay measures basal glycolysis and glycolysis capacity upon blockage of mitochondrial ATP production using respiratory modulators.
• Non-glycolytic acidification is assessed through glucose starvation.
• Compounds are reconstituted directly in assay medium – eliminating the need for DMSO or other solvents.
• Kits are rigorously tested and validated for every lot with cells to deliver consistent and robust results – eliminating the cost associated with failed experiments.
• Data analysis tool, the Seahorse XF Glycolysis Stress Test Report Generator, is provided to simplify data processing by automatically calculating the key parameters of your samples.
• Download Seahorse XF Glycolysis Stress Test Report Generator.